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Promega powerplex y23 system
Powerplex Y23 System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/powerplex+-y+system/powerplex+16+system/pmc12205656-40-40-43
Average 90 stars, based on 1 article reviews
powerplex y23 system - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Northern Blot:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. In this study, we analyze 514 Y-chromosomal haplotypes from three Kyrgyz subpopulations— Northern East Kyrgyzstan ( N = 134), Northern West Kyrgyzstan ( N = 183), and Southern Kyrgyzstan ( N = 197) —genotyped using 23 Y-STR markers with the PowerPlex Y23 System (Promega, USA). ..

Sterility:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Extraction:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Concentration Assay:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Spectrophotometry:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Amplification:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Polymerase Chain Reaction:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Electrophoresis:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Polymer:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

Software:

Article Title: Population data of 23 Y chromosome STR loci for Kyrgyz population from Kyrgyzstan
Article Snippet: .. Data collection , Samples were collected from healthy male volunteers of the Kyrgyz population using the Oragene DNA Self-Collection Kit (OG-500, DNA Genotek, Canada) for saliva and EDTA-coated sterile vacutainers (BD Vacutainer®, Becton Dickinson, USA) for blood. Each participant was verified to be unrelated to others in the study up to the third generation. DNA was extracted from saliva using the prepIT-L2P kit (DNA Genotek, Canada) and from blood using phenol-chloroform extraction preceded by proteinase K digestion. The quality and concentration of the DNA were assessed using the NanoDrop One Spectrophotometer and Qubit 2.0 Fluorometer (both from Thermo Fisher Scientific, USA). The DNA was then amplified by PCR using the PowerPlex Y23 System (Promega, USA) in a SimpliAmp Thermal Cycler (Thermo Fisher Scientific, USA). The PCR products were separated by electrophoresis using the WEN Internal Lane Standard 500 (Promega, USA) in Hi-Di Formamide on an Applied Biosystems 3500 genetic analyzer with an 8 capillary array and POP-4 polymer, along with Cathode and Anode buffers (all of them from Thermo Fisher Scientific, USA). The analysis of STR alleles in the electropherograms was conducted using the GeneMapper IDx v.1.6 software. Subsequent data analyses were performed utilizing Microsoft Office Excel, the Arlequin software version 3.5.2.2, STRAF software version 2.1.5, NevGen software version 1.0 and AMOVA&MDS tool from YHRD (Y-chromosome haplotype research database).. ..

other:

Article Title: Olaparib synergy screen reveals Exemestane induces replication stress in triple-negative breast cancer.
Article Snippet: All cell lines used in this study were authenticated within the past 3 years using short tandem repeat (STR) profiling with the PowerPlex 18D System (Promega, Madison, WI, USA), following the manufacturers’ protocols.

Article Title: The October 7 forensic DNA identification operation: Lessons learned from the management of the largest mass causality event in Israel.
Article Snippet: Correspondence Nurit Bublil, Israel National Center of Forensic Medicine 67 BenZvi Rd., P.O.. Box 8495 Tel Aviv, Israel.. Email: nurit.bublil@forensic.health.gov.il Abstract On October 7, 2023, the Hamas terrorist organization launched a surprise, violent attack on Israel, resulting in the largest mass casualty event in the country's history.

Article Title: Gut Microbial Similarity Analysis in Mono and Dizygotic Twins Discordant for Down Syndrome
Article Snippet: Genotyping by microsatellite analysis of 27 different short tandem repeat (STR) markers was performed using the PowerPlex® Fusion 6C System (Promega).

Article Title: Method for determining if a tumor has a mutation in a microsatellite
Article Snippet: Microsatellite PCR Genotyping For mononucleotide repeat instability, the PowerPlex MSI analysis system v1.2 (Promega) was used to test five markers following the manufacturer's protocol.



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